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bluescript ii ks+ vector  (Agilent technologies)


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    Structured Review

    Agilent technologies bluescript ii ks+ vector
    Bluescript Ii Ks+ Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bluescript+ii+ks++vector/pmc03606092-114-33-37
    Average 90 stars, based on 1 article reviews
    bluescript ii ks+ vector - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Cloning and initial analysis of the human multidrug resistance-related MVP/LRP gene promoter.
    Article Snippet: t M g c m i t S ( 2 t i s The lung resistance-related protein (LRP) was idenified as the human major vault protein (MVP), and is verexpressed in various multidrug-resistant cancer ell lines and clinical samples.. We characterized DNA equences upstream to the transcription initiation ite of the MVP gene in the human non-small cell lung ancer cell line SW-1573.. A 1.9-kb and a shortened .7-kb fragment of the 5*-upstream genomic region how strong promoter activity in chloramphenicol cetyltransferase (CAT) reporter assays.

    Article Title: The Amino Acid Sequence of Rat Kidney 5-Oxo-L-Prolinase Determined by cDNA Cloning
    Article Snippet: .. Recombinant l DNA purification was performed as described (13). cDNA clones were subcloned into a Bluescript II KS vector (Stratagene) and sequenced by the dideoxy chain termination method (14) with Sequenase (United States Biochemical Corp.) using T3, T7 primers or specific internal primers. ..

    Article Title: Protein factors in Blastocladiella emersonii cell extracts recognize similar sequence elements in the promoters of the genes encoding cAMP-dependent protein kinase subunits.
    Article Snippet: Blastocladiella emersonii contains a single cAMP-dependent protein kinase (PKA), which is similar to the mammalian type II isoforms.. Its activity is regulated during development by changes in the levels of the catalytic (C) and regulatory (R) subunits, which occur in parallel with changes in levels of the corresponding mRNAs, suggesting coordinate transcriptional control of the expression of both subunits.. Both R and C mRNA levels are low in vegetative cells, rise sharply during sporulation and decrease to basal levels again after germination.

    Article Title: Mouse retinol binding protein gene: cloning, expression and regulation by retinoic acid.
    Article Snippet: A full-length cDNA clone encoding the retinol binding protein (RBP) was isolated from a mouse liver cDNA library by hybridization screening.. The nucleotide sequence of murine RBP is 85 and 95% homologous to that of human and rat RBP, respectively, with a deduced amino acid sequence ≥ 83% homologous to both species.. Analysis of the tissue expression pattern of RBP mRNA in the female mouse indicated relatively abundant expression in the liver, with lesser amounts in extrahepatic tissues including adipose, kidney, spleen and uterus, suggesting that these tissues may have a significant role in retinol homeostasis.

    Clone Assay:

    Article Title: Comparison of Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus viral load in peripheral blood mononuclear cells and oral fluids of HIV-negative individuals aged 3-89 years from Uganda.
    Article Snippet: .. ERV-3 was cloned into Bluescript II KS vector (Stratagene, La Jolla, CA, USA) KSHV K6 cloned using PCR Topo II vector, Topo TA Cloning kit, Invitrogen, K 4600-40. ..

    Article Title: Comparison of Epstein–Barr virus and Kaposi’s sarcoma-associated herpesvirus viral load in peripheral blood mononuclear cells and oral fluids of HIV-negative individuals aged 3–89 years from Uganda
    Article Snippet: .. ERV-3 was cloned into Bluescript II KS vector (Stratagene, La Jolla, CA, USA) KSHV K6 cloned using PCR Topo II vector, Topo TA Cloning kit, Invitrogen, K 4600-40. ..

    Article Title: The Amino Acid Sequence of Rat Kidney 5-Oxo-L-Prolinase Determined by cDNA Cloning
    Article Snippet: .. Recombinant l DNA purification was performed as described (13). cDNA clones were subcloned into a Bluescript II KS vector (Stratagene) and sequenced by the dideoxy chain termination method (14) with Sequenase (United States Biochemical Corp.) using T3, T7 primers or specific internal primers. ..

    Article Title: Protein factors in Blastocladiella emersonii cell extracts recognize similar sequence elements in the promoters of the genes encoding cAMP-dependent protein kinase subunits.
    Article Snippet: Blastocladiella emersonii contains a single cAMP-dependent protein kinase (PKA), which is similar to the mammalian type II isoforms.. Its activity is regulated during development by changes in the levels of the catalytic (C) and regulatory (R) subunits, which occur in parallel with changes in levels of the corresponding mRNAs, suggesting coordinate transcriptional control of the expression of both subunits.. Both R and C mRNA levels are low in vegetative cells, rise sharply during sporulation and decrease to basal levels again after germination.

    Polymerase Chain Reaction:

    Article Title: Comparison of Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus viral load in peripheral blood mononuclear cells and oral fluids of HIV-negative individuals aged 3-89 years from Uganda.
    Article Snippet: .. ERV-3 was cloned into Bluescript II KS vector (Stratagene, La Jolla, CA, USA) KSHV K6 cloned using PCR Topo II vector, Topo TA Cloning kit, Invitrogen, K 4600-40. ..

    Article Title: Comparison of Epstein–Barr virus and Kaposi’s sarcoma-associated herpesvirus viral load in peripheral blood mononuclear cells and oral fluids of HIV-negative individuals aged 3–89 years from Uganda
    Article Snippet: .. ERV-3 was cloned into Bluescript II KS vector (Stratagene, La Jolla, CA, USA) KSHV K6 cloned using PCR Topo II vector, Topo TA Cloning kit, Invitrogen, K 4600-40. ..

    Article Title: Mouse retinol binding protein gene: cloning, expression and regulation by retinoic acid.
    Article Snippet: A full-length cDNA clone encoding the retinol binding protein (RBP) was isolated from a mouse liver cDNA library by hybridization screening.. The nucleotide sequence of murine RBP is 85 and 95% homologous to that of human and rat RBP, respectively, with a deduced amino acid sequence ≥ 83% homologous to both species.. Analysis of the tissue expression pattern of RBP mRNA in the female mouse indicated relatively abundant expression in the liver, with lesser amounts in extrahepatic tissues including adipose, kidney, spleen and uterus, suggesting that these tissues may have a significant role in retinol homeostasis.

    TA Cloning:

    Article Title: Comparison of Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus viral load in peripheral blood mononuclear cells and oral fluids of HIV-negative individuals aged 3-89 years from Uganda.
    Article Snippet: .. ERV-3 was cloned into Bluescript II KS vector (Stratagene, La Jolla, CA, USA) KSHV K6 cloned using PCR Topo II vector, Topo TA Cloning kit, Invitrogen, K 4600-40. ..

    Article Title: Comparison of Epstein–Barr virus and Kaposi’s sarcoma-associated herpesvirus viral load in peripheral blood mononuclear cells and oral fluids of HIV-negative individuals aged 3–89 years from Uganda
    Article Snippet: .. ERV-3 was cloned into Bluescript II KS vector (Stratagene, La Jolla, CA, USA) KSHV K6 cloned using PCR Topo II vector, Topo TA Cloning kit, Invitrogen, K 4600-40. ..

    Recombinant:

    Article Title: The Amino Acid Sequence of Rat Kidney 5-Oxo-L-Prolinase Determined by cDNA Cloning
    Article Snippet: .. Recombinant l DNA purification was performed as described (13). cDNA clones were subcloned into a Bluescript II KS vector (Stratagene) and sequenced by the dideoxy chain termination method (14) with Sequenase (United States Biochemical Corp.) using T3, T7 primers or specific internal primers. ..

    DNA Purification:

    Article Title: The Amino Acid Sequence of Rat Kidney 5-Oxo-L-Prolinase Determined by cDNA Cloning
    Article Snippet: .. Recombinant l DNA purification was performed as described (13). cDNA clones were subcloned into a Bluescript II KS vector (Stratagene) and sequenced by the dideoxy chain termination method (14) with Sequenase (United States Biochemical Corp.) using T3, T7 primers or specific internal primers. ..

    Amplification:

    Article Title: Mouse retinol binding protein gene: cloning, expression and regulation by retinoic acid.
    Article Snippet: A full-length cDNA clone encoding the retinol binding protein (RBP) was isolated from a mouse liver cDNA library by hybridization screening.. The nucleotide sequence of murine RBP is 85 and 95% homologous to that of human and rat RBP, respectively, with a deduced amino acid sequence ≥ 83% homologous to both species.. Analysis of the tissue expression pattern of RBP mRNA in the female mouse indicated relatively abundant expression in the liver, with lesser amounts in extrahepatic tissues including adipose, kidney, spleen and uterus, suggesting that these tissues may have a significant role in retinol homeostasis.



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